体外培养法研究三聚氰胺的降解规律及其对瘤胃发酵参数的影响

doi:10.11843/j.issn.0366-6964.2014.04.011

Abstract

Abstract:

This experiment was conducted to investigate the effects of melamine on ruminal fermentation parameters in vitro.Melamine was supplemented at graded levels：0，2，10，50，250，1 250 mg•L^-1，respectively.Incubation mediums were taken after incubation of 0，1，2，4，6，8，12，24 and 48 h，respectively.Melamine，total fatty acid (TVFA)，acetic acid，propionic acid，butyric acid，valeric acid，isobutyric acid，isovaleric acid and ammonia nitrogen concentration were determined.The result showed that melamine supplementation resulted in no change in MEL degradation during 48 h incubation （P>0.05） but decreased dry matter degradability (DMD)（P<0.05）.With MEL supplementation at the level of 1 250 mg•L^-1，molar proportion of propionic acid decreased（P<0.05）while ratio of acetic acid to propionic acid increased（P<0.05）.Molar proportion of butyric acid increased（P<0.05）with MEL supplementation.Melamine showed an inhibitory effect on ruminal microbial fermentation and might not be effectively used by ruminal microbes.Therefore，MEL could not be used as a non-protein nitrogen source for feeding ruminant animals.

CLC Number:

S823
S815.4

JIANG Cheng-gang，YAN Gui-long，TU Yan，ZHANG Nai-feng，DIAO Qi-yu. The Study on Degradability of Melamine and Effcet of Fermentation Characteristics in vitro[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(4): 578-586.

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References

［1］MAST R W，JEFFCOAT A R，SADLERB M，et al.Metabolism，disposition and excretion of ［14C］melamine in male Fischer 344 rats［J］.Food Chem Toxicol，1983，21(6)：807-810.
［2］SUGITA T H，ISHIWATA A，MAEKA W A.Intestinal absorption and urinary excretion of melamine in male Wistar rats［J］. J Food Hygienic Soc Jap，1991，32(5)：439-443.
［3］金鑫,张艳,李胜利,等.三聚氰胺在奶牛体内代谢规律的研究［J］.动物营养学报,2010,22(6):1703-1708.
［4］CRUYWAGEN C W，VAN DE VYVER W F，STANDER M A.Quantification of melamine absorption，distribution to tissues and excretion by sheep［J］.J Anim Sci，2011,89(7):2164-2169.
［5］SHEN J S，WANG J Q，WEI H Y，et al.Transfer efficiency of melamine from feed to milk in lactating dairy cows fed with different doses of melamine［J］.J Dairy Sci，2010，93(5)：2060-2066.
［6］NEWTON G L，UTLEY P R.Melamine as a dietary nitrogen source for ruminants［J］.J Anim Sci，1978，47(6)：1338-1344.
［7］庄宏,杨红建,曹兵海,等.不同三聚氰胺添加量对高精混合饲料体外瘤胃发酵理化指标的影响［J］.中国农业大学学报,2010，(1)：45-49.
［8］孟祥坤,杨红建,曹兵海,等.添加三聚氰胺对高粗料日粮短期人工瘤胃发酵理化指标的影响［J］.中国畜牧杂志,2010，(9)：52-55.
［9］JUTZI K，COOK A M，HUTTER R.The degradative pathway of the s-triazine melamine.The steps to ring cleavage［J］.Biochem J，1982，208(3)：679-684.
［10］SHELTON D R，KARNS J S，MCCARTY G W，et al.Metabolism of Melamine by Klebsiella terragena［J］.Appl Environ Microbiol，1997，63(7)：2832-2835.
［11］TILLEY J M A，TERRY R A.A two-stage technique for the in vitro digestion of forage crops［J］.J Br Grassland Soc，1963，18：104-111.
［12］MCDOUGALL E I.Studies on ruminant saliva.1.The composition and output of sheep’s saliva［J］.Biochem J，1948，43(1)：99-109.
［13］BRODERICK G A，KANG J H.Automated simultaneous determination of ammonia and total amino acids in ruminal fluid and in vitro media［J］. J Dairy Sci，1980，63(1)：64-75.
［14］代俊芳,孟庆翔,周振明,等.硝态氮添加水平对体外瘤胃发酵和微生物合成效率的影响［J］.中国农业科学,2010,43(16):3418-3424.
［15］武书庚,高春起,岳洪源,等.三聚氰胺在鸭蛋中的沉积和消除规律的研究［J］.动物营养学报,2010,22(2):416-423.
［16］张丽英.饲料分析及饲料质量检测技术［M］.3版.北京:中国农业大学出版社,2007:48-52.
［17］COOK A M，HUTTER R.s-Triazines as nitrogen sources for bacteria［J］.J Agric Food Chem，1981，29(6)：1135-1143.
［18］WANG Z，CHEN L，AL-KASIR R，et al.In vitro toxicity of melamine against Tetrahymena pyriformis cells［J］.J Vet Sci，2011，12(1)：27-34.
［19］ISHIWATA H，SUGITA T，KOZAKI M，et al.Inhibitory effects of melamine on the growth and physiological activities of some microorganisms［J］. J Food Hygienic Soc Jap，1991，32(5)：408-413.
［20］BERGMAN E N.Energy contributions of volatile fatty acids from the gastrointestinal tract in various species［J］.Physiol Rev，1990，70(2)：567-590.
［21］ESKELAND B，PFANDER W H，PRESTON R L.Intravenous energy infusion in lambs：effects on nitrogen retention，plasma free amino acids and plasma urea nitrogen［J］.Br J Nutr，1974，31(2)：201-211.
［22］KRISTENSEN N B，HARMON D L.Effect of increasing ruminal butyrate absorption on splanchnic metabolism of volatile fatty acids absorbed from the washed reticulorumen of steers［J］.J Anim Sci，2004，82(12)：3549-3559.
［23］MIETTINEN H，HUHTANEN P.Effects of the ratio of ruminal propionate to butyrate on milk yield and blood metabolites in dairy cows［J］.J Dairy Sci，1996，79(5)：851-861.
［24］HARMON D L，GROSS K L，KREHBIEL C R.et al.Influence of dietary forage and energy intake on metabolism and acyl-CoA synthetase activity in bovine ruminal epithelial tissue［J］. J Anim Sci，1991，69(10)：4117-4127.
［25］LIU Q，WANG C，HUANG Y X，et al.Effects of isovalerate on ruminal fermentation，urinary excretion of purine derivatives and digestibility in steers［J］.J Anim Physiol Anim Nutr (Berl)，2009，93(6)：716-725.

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The Study on Degradability of Melamine and Effcet of Fermentation Characteristics in vitro

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